Antonio Ribeiro
Universidade de Coimbra, Portugal
Title: Layer-By-Layer Self-Assembly Nanoparticles Designed For Resveratrol Delivery
Biography
Biography: Antonio Ribeiro
Abstract
Resveratrol (RSV) is a non-flavonoid polyphenol which exhibits a wide array of beneficial multi-target biological effects in preclinical studies. Besides this evidence, the far-reaching application of RSV is strongly hampered due to a low favourable pharmacokinetics behaviour attributed to its poor water solubility. This way, an unmet need to develop proper RSV delivery systems is posed, aiming for the enhancement of its bioavailability in vivo. An advanced formulation strategy able to surpass this limitation is nanotechnology, whereby Layer-by-Layer (LbL) self-assembly nanoparticles assume particular emphasis. LbL self-assembly is an encapsulation technique which is built on the sequential assembly of oppositely charged polyelectrolytes upon charged low soluble drug nanoparticles’ surface. Tunned nanocapsule-type constructions at nanoscale are obtained, fundamentally comprising a drug nanocore which is covered by a poleyelectrolyte-based multilayered nanoshell. Herein, a LbL self-assembly technique coupled with a washless polyelectrolyte approach was developed. Aqueous RSV nanoparticles composed of distinct bilayers of polyallylamine hydrochloride (PAH) as the polycation, and, dextran sulphate (DS) as the polyanion, were performed (including, 2.5 (RSV-(PAH/DS)2.5), 5.5 (RSV-(PAH/DS)5.5) and 7.5 (RSV-(PAH/DS)7.5) bilayers). Homogenous particle size distributions with 150-250 nm were obtained. The most complex formulation, composed 7.5-bilayers, evidenced 219 ± 1 nm and 0.17 of polydispersity index; high electrical surface of +31 ± 0.5 mV; and a high drug content of 92 ± 2%. The modulation of the composition of the coating shell allowed the control of the RSV release pattern. Owing to such, these LbL nanoparticles are considered as a promising vehicle for the delivery of RSV.